Microbiology lab report on Escherichia coli and Staphylococcus aureus.

Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this research laboratory experiment 2 diametral types of bacteria, Escherichia coli and Staphylococcus aureus, were grown severally and mixed on multiple diametrical types of nutrient nutrient nutrient agar-agar in order to bump the vary morphologies deep master the colonies. Resulting data was analyzed to furnish understanding of the using up of differing culture media and conditions for bacterial ontogenesis. RESULTSFour different agar types were use in this experiment. The first ( alimental) allow fored for proceeds of both(prenominal) E. coli and S. aureus. The second agar employ (MKL) inhibited the developing of S. aureus and allowed the branch of E. coli. The third (ash tree) kick ined increase of both bacterial species. The quaternary (mannitol) was found to inhibit growth of E. coli (Table 1). Table 1. feature of growth within the four types of agar. G = growth, NG = no growth. E. coliS. aureusMixed cultureNutrientGGGMKLGNGG alterGGGmannitolNGGGChanges in colour within the sampled cultures were similarly noted. No variety shows occurred within the Nutrient agar. The MKL agar exhibited a vividness change with E. coli and the mixed culture. The agar changed from red to purple, and a homely border was present adjoin the colonies that exhibited growth on the dish. The ASH agar did not yield a color change. The Mannitol agar exhibited a change with S. aureus and the mixed culture, but no change with E. coli (Table 2). Table 2.

Indication of color change within the four types of agar. E. coliS. aureusMixed cultureNutrientTransparentTransparentTransparentMKLRed to PurpleRedRed to PurpleASHRedRedRedMannitolRedRed to YellowRed to YellowCONCLUSIONS AND DISCUSSIONTwo noticeable changes occurred during this experiment. First, two of the twelve inoculated Petri dishes yielded no growth. In order to explain these occurrences, we mustiness first examine the differing properties of the types of agar used. Nutrient agar is enriched to allow growth of a vernacular variety... If you want to get a full essay, order it on our website: Orderessay

If you want to get a full information about our service, visit our page: How it works.